SODIUM DODECYL SULFATE (SDS)

 

 

 

151-21-3

 

 

SDS; Lauryl sulfate sodium salt; Dodecyl sulfate sodium salt; Dodecyl sodium sulfate; Sodium lauryl sulfate; Sulfuric acid monododecyl ester sodium salt

SDS is an anionic detergent typically used to solubilize and denature proteins for electrophoresis. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size. The critical micelle concentration (CMC) in pure water at 25°C is 8.27 mM, and the aggregation number at this concentration is usually considered to be about 62. The micelle ionization fraction (α) is around 0.3 (or 30%).

Used to solubilize and denature proteins for SDS-PAGE. SDS is used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material, used to aid in lysing cells during DNA extraction, for unraveling proteins in SDS-PAGE and in preparing proteins for electrophoresis in the SDS-PAGE technique. This compound works by disrupting non-covalent bonds in the proteins, denaturing them, and causing the molecules to lose their native shape (conformation). It is an anionic surfactant used in many cleaning and hygiene products. Aqueous solutions of SDS are used for dispersing or suspending nanotubes. SDS is also used in the analysis of hemoglobin where the hydrophobic group of SDS acts upon the globin subunit, causing a conformational change and the hydrophillic group of SDS then binds with the oxidized iron subunit, producing a stable reaction product which can then be analyzed, giving a hemoglobin value which is used as part of a complete blood count.SDS was used as hybridization buffer for non-microfluidic NanoGene assay (Ahjeong Son,(2012),Development of an in-situ capable method for detecting pathogenic bacteria in the alabama water supplies – phase i & ii,Synopsis)