Protocol: SDS Page Gel Recipe
SDS Page Gel (Preparing SDS-Page Gels)
An anionic detergent typically used to solubilize and denature
proteins for electrophoresis. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size.
Typical Working Concentration: > 10 mg SDS/mg protein
Typical Buffer Compositions:
Typical Working Concentration: > 10 mg SDS/mg protein
Typical Buffer Compositions:
SDS Electrophoresis Gel Running Buffer:
|
Component |
Composition (g/L) |
Molarity or % |
|
3.035 |
0.025 M | |
|
14.1 |
0.192 M | |
|
1 |
0.1% | |
|
pH 8.3 |
 |
|
SDS Electrophoresis Gel Sample Solubilization Buffer
|
Component |
Composition (g/L) |
Molarity or % |
|
15.16 |
0.125 M | |
|
20.0 |
2.0% | |
|
100 |
10.0% | |
|
0.2 |
0.02% |
Titrated to pH 8.0 with HCl
